Figure 6.: Methods Comparison and Correlation Studies for Absolute CD4 Counts in 61 Adults in Botswana

Bland–Altman methods comparison plot comparing absolute CD4 cells per microliter of whole blood obtained by the microchip system as compared to standard four-color flow cytometry processed in parallel on a FACSCalibur in 61 HIV-infected adult participants. There is a proportional bias of -50 cells/µl relative to flow cytometry. Grey line indicates zero bias. Red lines indicate upper and lower 95% limits of agreement.

From: Rodriguez WR, Christodoulides N, Floriano PN, Graham S, Mohanty S, et al. (2005) A Microchip CD4 Counting Method for HIV Monitoring in Resource-Poor Settings. PLoS Med 2(7): e182