Figure 5.: Representative Processed Data Images from Three Participants in Botswana

The participants included (A) a 31-y-old woman with a CD4 count of 83 cells/µl by flow cytometry; (B) a 33-y-old woman with a CD4 count of 271 cells/µl by flow cytometry; and (C) a 5-mo-old infant with an absolute CD4 count of 2,098 cells/µl and a CD4:CD8 ratio of 1.80 by flow cytometry. In these images, CD3+CD8+ T cells appear red, monocytes appear green, and CD3+CD4+ T cells appear yellow. Each image reflects 0.18 µl of whole blood.

From: Rodriguez WR, Christodoulides N, Floriano PN, Graham S, Mohanty S, et al. (2005) A Microchip CD4 Counting Method for HIV Monitoring in Resource-Poor Settings. PLoS Med 2(7): e182