Figure 2.: The Membrane Flow Cell Selectively Captures Lymphocytes and Provides for the Removal of Red Blood Cells without Sample Processing

(A) A whole blood sample collected atop a glass slide and imaged by a scanning electron microscope reveals the overabundance of red blood cells in the sample.

(B) A whole blood sample processed through the flow cell reveals that lymphocytes are captured on the membrane support while red blood cells are largely excluded from within the cell. Arrows indicate red blood cells passing through the membrane.

(C) Fluorescent antibody stain specific for a lymphocyte marker is used to visualize captured lymphocytes within the flow cell in a representative single-color data image.

 

From: Rodriguez WR, Christodoulides N, Floriano PN, Graham S, Mohanty S, et al. (2005) A Microchip CD4 Counting Method for HIV Monitoring in Resource-Poor Settings. PLoS Med 2(7): e182